Job type
Thesis
Job Service
Centre for Education, Research and Innovation in Energy and Environment
Job Location
Douai

Context

Scientific field: Biology and Engineering
Supervisor: CODDEVILLE Patrice, Director of the Research
Co-supervisor: GMYR Valery, assistant professor
Laboratories: Centre for Education, Research and Innovation in Energy and Environment (IMT Lille Douai) and Diabetes Translational Research Laboratory (Lille Faculty of Medicine Research Unit)
Related research project: I-site ULNE, Labex CaPPA and Labex EGID,
Funding: I-site ULNE

Type 1 diabetes is an autoimmune disorder that causes destruction of insulin-producing α-cells, which are specific human pancreas endocrine glands. Exogenous insulin is often required to treat the disease. The study of pancreatic hormonal secretions (insulin, glucagon, somatostatin) is a necessary prerequisite for the development of new cellular therapies. The laboratory for translational research on diabetes (INSERM U1190) in collaboration with the Energy Environment Research Centre and FabLab of IMT Lille Douai have developed a perifusion chamber that allows in vitro measurement of the hormonal secretion of islets of Langerhans (IL) subjected to secretagogues. Perifusion systems has been transposed and can therefore be used as an excellent test for quality control of an IL preparation. This technique is operational but still very manual so it’s necessary to make changes in the perifusion system in order to obtain numerous parameters such as oxygen consumption for example. In a context where this therapy is likely to expand (clinical treatment of type 1 diabetes by allograft recognized by the French health insurance in 2021), it appears imperative to perfect a dynamic evaluation test of IL that can meet scientific and clinical expectations. The aim of this project is to develop a perifusion chamber that combines the fineness of analysis of hormonal secretions with the, integration of physiological measurements during a cellular stimulation experiment. In order to carry out this project, it is necessary to bring together several skills, such as engineering, metrology, biological knowledge of the islets of Langerhans and clinical expertise.

The use of digital tools and equipment to optimize the design and manufacture of a new perifusion chamber that is more functional and scalable with the addition of parameters is essential. The FabLab of IMT Lille Douai will support the design of new perifusion chamber prototypes and the flow and pressure calculations applied within the reaction chambers. These chambers will be made using the complete chain of design, from 3D drawing (SOLIDWORKS of Dassault System) to laser cutting and 3D printing. Laboratory U1190 will contribute its expertise by isolating IL from human and murine pancreas and performing physiological stimulation tests. The PhD student will be jointly supervised by a tutor from each laboratory and will carry out this work at IMT Lille Douai and at the Faculty of Medicine in Lille.

The doctoral student will be able to benefit from the help of the engineering students of IMT Lille Douai through student projects. The aim of this work will be to provide medical teams and research laboratories with a tool to measure, in a known environment, the hormonal secretions of a pancreatic cell preparation and to determine the factors that can influence their physiological regulation. This work may lead to the submission of a patent. This strong collaboration between clinical research and engineering will allow the development of new ways for exploration in the therapeutic treatment of diabetes

Conditions

Planned recruitment date: Ongoing recruitment via Isite (13th April)

Contact

CODDEVILLE Patrice, Director of the Research
GMYR Valery, assistant professor

Candidate to offer